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Fig. 9 | Reproductive Biology and Endocrinology

Fig. 9

From: Divergent activity of the gonadotropin-releasing hormone receptor gene promoter among genetic lines of pigs is partially conferred by nuclear factor (NF)-kB, specificity protein (SP)1-like and GATA-4 binding sites

Fig. 9

Mutations of the NF-κB, SP1 or both elements reduce activity of Meishan reporter constructs. Luciferase (LUC) reporter vectors containing either the native, full-length Meishan GnRHR gene promoter (−M5118pGL3), block replacement mutations of the NF-κB (−MμNF-κBpGL3), SP1 (−MμSp1pGL3) or a combination of the two (−Mμ1240pGL3) binding sites within the context of −M5118pGL3, or promoterless control (pGL3) were transiently transfected into αT3-1 cells. Unique letters indicate means that are significantly different from one another (P < 0.05)

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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