Fig. 1
From: MicroRNA-21 and PDCD4 expression during in vitro oocyte maturation in pigs
MIR21 expression is significantly increased in cumulus cells and oocytes during in vitro maturation. a RT-qPCR analysis for MIR21 and PDCD4 mRNA in GV stage and MII arrested oocytes (n = 4). b RT-qPCR analysis for MIR21 in cumulus cells isolated from GV stage and in vitro matured oocytes (n = 4). c PDCD4 Western blot analysis of GV stage and MII arrested oocytes. Pixel intensity was quantified with ImageJ and demonstrates a decrease in PDCD4 protein abundance in MII oocytes compared with GV oocytes (n = 3). d Representative Western blot of PDCD4 protein detection in oocytes representing the 52 KDa band. a,bMeans ± SEM with different superscripts are different (P < 0.05)