Fig. 3

Micro-well culturing POU5F1-GFP expressing embryos. 2PN zygotes (n = 107) expressing Pou5f1-GFP were cultured individually in control medium with micro-well method (one embryo/10 μL/well) and monitored for morphology and GFP expression up to 96 h. Developmental progress of individual embryos was shown as a hierarchical diagram. Pou5f1-GFP embryos were evaluated at 48 h and grouped as EFI 0–1 (score 0–1) or EFI 2–3 (score 2–3). Their successive development was monitored and graded at 96 h with detection of POU5F1-GFP and divided into two groups: expanded and hatching/hatched blastocysts (E/H, yellow) or ≤ blastocysts (white)