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Fig. 3 | Reproductive Biology and Endocrinology

Fig. 3

From: Flutamide induces alterations in the cell-cell junction ultrastructure and reduces the expression of Cx43 at the blood-testis barrier with no disturbance in the rat seminiferous tubule morphology

Fig. 3

a-c Analyses of Cx43 gene expression in testes of control and flutamide-treated rats. Representative RT-PCR (a), quantitative real-time PCR (b), and Western blot (c). As an internal control, the GAPDH mRNA level was measured in the samples. N1 – negative control without cDNA template. N2 – negative control without reverse-transcribed RNA. Relative quantification (RQ) is expressed as mean ± SD. The relative level of Cx43 protein normalized to β-actin which served as an internal protein loading control. Data obtained from three separated analysis are expressed as mean ± SD. Significant differences from control values are denoted as *p < 0.05. Control (n = 6) and flutamide (n = 6) group

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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