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Table 2 Effect of BMP-4 in IVG medium on the nuclear maturation and diameter of IVG oocytes

From: Effect of bone morphogenetic protein-4 on in vitro growth, steroidogenesis and subsequent developmental competence of the oocyte-granulosa cell complex derived from bovine early antral follicles

Oocyte BMP-4 (ng/ml) No. of oocytes cultured (replicates) Mean oocyte diameter (μm) % of nuclear status (no.)
Before IVG After IVM M I A I/ T I M II
In vitro grown 0 33c (4) 98.9 ± 3.4 116.9 ± 4.1b 6.5 (2) 0 93.5 (29)
10 29 (4) 99.4 ± 3.0 117.7 ± 3.5b 10.3 (3) 0 89.7 (26)
50 30 (4) 99.1 ± 3.1 116.8 ± 2.9b 3.3 (1) 3.3 (1) 93.4 (28)
In vivo grownd - 32 (3) - 120.2 ± 3.1a 6.3 (2) 0 93.8 (30)
  1. Abbreviations: M I metaphase I, A I/T I anaphase I/telophase I, M II metaphase II
  2. abValues with different superscripts in the same column are significantly different (p < 0.05)
  3. cFor the evaluation of nuclear maturation, 31 oocytes in the 0 ng/ml BMP-4 treated group were used because 2 oocytes were lost during fixation
  4. dOocytes collected from antral follicles (2–8 mm in diameter) served as in vivo-derived controls