Effect of bone morphogenetic protein-4 on in vitro growth, steroidogenesis and subsequent developmental competence of the oocyte-granulosa cell complex derived from bovine early antral follicles

Table 2 Effect of BMP-4 in IVG medium on the nuclear maturation and diameter of IVG oocytes

Oocyte	BMP-4 (ng/ml)	No. of oocytes cultured (replicates)	Mean oocyte diameter (μm)		% of nuclear status (no.)
Oocyte	BMP-4 (ng/ml)	No. of oocytes cultured (replicates)	Before IVG	After IVM	M I	A I/ T I	M II
In vitro grown	0	33^c (4)	98.9 ± 3.4	116.9 ± 4.1^b	6.5 (2)	0	93.5 (29)
	10	29 (4)	99.4 ± 3.0	117.7 ± 3.5^b	10.3 (3)	0	89.7 (26)
	50	30 (4)	99.1 ± 3.1	116.8 ± 2.9^b	3.3 (1)	3.3 (1)	93.4 (28)
In vivo grown^d	-	32 (3)	-	120.2 ± 3.1^a	6.3 (2)	0	93.8 (30)

Abbreviations: M I metaphase I, A I/T I anaphase I/telophase I, M II metaphase II
^abValues with different superscripts in the same column are significantly different (p < 0.05)
^cFor the evaluation of nuclear maturation, 31 oocytes in the 0 ng/ml BMP-4 treated group were used because 2 oocytes were lost during fixation
^dOocytes collected from antral follicles (2–8 mm in diameter) served as in vivo-derived controls

ISSN: 1477-7827