|
Oocyte
|
BMP-4 (ng/ml)
|
No. of oocytes cultured (replicates)
|
Mean oocyte diameter (μm)
|
% of nuclear status (no.)
|
|---|
|
Before IVG
|
After IVM
|
M I
|
A I/ T I
|
M II
|
|---|
|
In vitro grown
|
0
|
33c (4)
|
98.9 ± 3.4
|
116.9 ± 4.1b
|
6.5 (2)
|
0
|
93.5 (29)
|
|
10
|
29 (4)
|
99.4 ± 3.0
|
117.7 ± 3.5b
|
10.3 (3)
|
0
|
89.7 (26)
|
|
50
|
30 (4)
|
99.1 ± 3.1
|
116.8 ± 2.9b
|
3.3 (1)
|
3.3 (1)
|
93.4 (28)
|
|
In vivo grownd
|
-
|
32 (3)
|
-
|
120.2 ± 3.1a
|
6.3 (2)
|
0
|
93.8 (30)
|
- Abbreviations: M I metaphase I, A I/T I anaphase I/telophase I, M II metaphase II
-
abValues with different superscripts in the same column are significantly different (p < 0.05)
-
cFor the evaluation of nuclear maturation, 31 oocytes in the 0 ng/ml BMP-4 treated group were used because 2 oocytes were lost during fixation
-
dOocytes collected from antral follicles (2–8 mm in diameter) served as in vivo-derived controls
