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Table 2 Effect of BMP-4 in IVG medium on the nuclear maturation and diameter of IVG oocytes

From: Effect of bone morphogenetic protein-4 on in vitro growth, steroidogenesis and subsequent developmental competence of the oocyte-granulosa cell complex derived from bovine early antral follicles

Oocyte

BMP-4 (ng/ml)

No. of oocytes cultured (replicates)

Mean oocyte diameter (μm)

% of nuclear status (no.)

Before IVG

After IVM

M I

A I/ T I

M II

In vitro grown

0

33c (4)

98.9 ± 3.4

116.9 ± 4.1b

6.5 (2)

0

93.5 (29)

10

29 (4)

99.4 ± 3.0

117.7 ± 3.5b

10.3 (3)

0

89.7 (26)

50

30 (4)

99.1 ± 3.1

116.8 ± 2.9b

3.3 (1)

3.3 (1)

93.4 (28)

In vivo grownd

-

32 (3)

-

120.2 ± 3.1a

6.3 (2)

0

93.8 (30)

  1. Abbreviations: M I metaphase I, A I/T I anaphase I/telophase I, M II metaphase II
  2. abValues with different superscripts in the same column are significantly different (p < 0.05)
  3. cFor the evaluation of nuclear maturation, 31 oocytes in the 0 ng/ml BMP-4 treated group were used because 2 oocytes were lost during fixation
  4. dOocytes collected from antral follicles (2–8 mm in diameter) served as in vivo-derived controls