Fig. 4

D2-downstream pathway inhibitors do not affect LGC VEGF secretion in vitro. a Conventional pathways through which D2 signals are transduced and the inhibitors commonly used to block them; PTX = pertussis toxin, WT = wortmannin, and PMA = phorbol 12-myristate 13-acetate (circles indicate inhibitory actions). Each of these compounds inhibits one of the D2-downstream pathways. b VEGF secretion levels measured by ELISA (expressed as picograms per milliliter) from LGCs (n = 6) cultured with hCG in the presence or absence of Cabergoline (Cb2) and PTX, WT, or PMA. Inhibitors of D2-downstream pathways did not reverse the effect of D2-ag on VEGF secretion. *p < 0.01, compared to the (control) D2-ag treated (blank bar) group