Fig. 3
From: Human sperm degradation of zona pellucida proteins contributes to fertilization
Obtaining of ubiquitinated recombinant human ZP proteins. Human ZP2, ZP3 and ZP4 proteins were expressed in Sf9 cell line under heat shock conditions followed by affinity purification. (a) The purity of rhZP proteins was examined by SDS-PAGE followed by staining with CBB. (b) The identity of purified proteins was determined by Western blot using anti-HSPZ for primary detection. (c) Ubiquitination levels of purified rhZP proteins were assessed by Western blot of same proteins using anti-Ub antibodies