Fig. 5
From: Nuclear factor of activated T-cell isoform expression and regulation in human myometrium
Impact of BAPTA-AM on NFATc2 gene expression in cultured (P2) pregnant human myometrial cells during a 6 h stretch period. A) NFATc2 expression in non-stretched control cells (t = 0 to t = 6 h) treated with BAPTA-AM (20 μM) (blue line) or vehicle control (0.1 % dimethyl sulfoxide) (black line). B) NFATc2 expression in 6 h stretched (25 % static strain) cells (t = 0 to t = 6 h) treated with BAPTA-AM (blue line) or vehicle control (black line). Data are expressed as mean ± SE log copy number [n =10, normalised to GeNorm normalisation factor (NF)]. In non-stretched cells there was a slight change in NFATc2 expression (# P < 0.05) from t = 0 h to t = 6 h in BAPTA-AM treated cells, but there was no significant difference in BAPTA-AM-treated versus vehicle control at t = 6 h. Both BAPTA-AM treated and vehicle control treated cells under stretch demonstrated a significant change in NFATc2 expression between t = 0 h to t = 6 h (##, P = 0.001) but the effect of stretch was significantly greater in cells in the absence of BAPTA-AM (** P < 0.001)