Fig. 1

Charaterization of Visf (A) and ADP (B) antiserum. 1. The sequence of the peptides used for immunisation is shown at the top followed by the corresponding Visf (A) or ADP (B) sequence in selected species. Red colour indicates difference from the peptide used for immunisation (Bos taurus peptide for adiponectin and Gallus gallus peptide for Visf). 2. Plasma and protein lysates from chicken AT and leg muscle (60 μg) were analysed with SDS-PAGE under reducing conditions. Immunoblotting was performed with pre-immune rabbit serum (PRE) or chicken Visf antiserum (left panel) or Bos taurus ADP antiserum (right panel) (POST) in the absence (−) or presence (+) of 20 μg/ml of the chicken Visf antibody (left panel) or the Bos taurus ADP antibody (right panel). The arrow on the right indicates the position of the Visf (left panel) or ADP (right panel) signal. 3. Visf (left panel) and ADP (right panel) protein expression by immunoblotting in leg muscle, AT and plasma in turkey, bovine and chicken species. The picture is representative of the lysates obtained on three different animals