Fig. 1

Comparison of yield and viability of purified cytotrophoblast cells among a variety of enzymatic degradation protocols. Yield (a) and viability (b) of purified cytotrophoblast cells using different enzymatic digestion protocols were assessed. Protocol 1: digestion three times in 0.25 % trypsin for 30 min each [10]; Protocol 2: digestion two times in 0.25 % trypsin for 10 min each [11]; Protocol 3: digestion in 0.125 % trypsin and 0.2 mg/ml DNase I for 45 min [14]; Protocol 4: digestion three times in 0.125 % trypsin and 0.2 mg/ml DNase I for 30 min each [15]; Protocol 5: digestion three times in 1 mg/ml Dispase II, 0.5 mg/ml collagenase I and 0.1 mg/ml DNase I for 15 min each; Protocol 6: digestion three times in 1 mg/ml Dispase II, 0.5 mg/ml collagenase I and 0.1 mg/ml DNase I for 20 min each; Protocol 7: digestion two times in 1 mg/ml Dispase II, 0.5 mg/ml collagenase I and 0.1 mg/ml DNase I for 30 min each. Data are presented as mean ± SD of six independent experiments