Fig. 2

Silencing of both LAT isoforms additively reduces System L transport activity. PHT cells were transfected with non-targeting scramble-siRNA (si-SCR) or LAT1-siRNA and LAT2-siRNA (si-LAT1/2). a Histograms illustrate relative protein expression of LAT1 and LAT2 following si-RNA treatments, N = 3. b System L amino acid transport measured after si-RNA treatments, N = 4. Mean of controls was assigned an arbitrary value of 1.0 and data represents fold change from si-SCR; mean + SEM. Student t-test, **P < 0.01, ****P < 0.0001 vs control (si-SCR)