Table 1 Semen parameters for the first evaluation and during the daily ejaculation period of 2 weeks. (Mean ± SEM, n = 6)

Abstinence, days

3-4

1

1

1

1

1

1

Volume, mL

2.5 ± 0.2

1.8 ± 0.4*

1.8 ± 0.3*

1.6 ± 0.2*

1.9 ± 0.3*

1.7 ± 0.4 *

1.9 ± 0.2*

Sperm concentration, 10⁶/mL

121.3 ± 35.5

80.3 ± 18.7

72.1 ± 18.1

66.1 ± 15.2

75.1 ± 22.6

80.4 ± 21.5

80.3 ± 19.2

Total sperm count, ×10⁶

276.8 ± 70.5

134.4 ± 46.6*

115.7 ± 27.8*

88.7 ± 16.2*

111.5 ± 23.6*

109.9 ± 27.3*

138.2 ± 31.4*

Total motility, %

53.83 ± 4.7

55.50 ± 10.2

54.0 ± 7.8

52.9 ± 7.0

58.5 ± 5.7

58.7 ± 4.4

51.70 ± 5.5

Progressive motility, %

47.0 ± 5.5

51 ± 11.3

43.0 ± 8.6

45.2 ± 8.0

46.6 ± 6.4

50.5 ± 5.0

40.8 ± 3.3

Vitality, %

60.2 ± 3.1

61.7 ± 8.8

53.4 ± 7.4

65.5 ± 5.3

60.2 ± 4.7

59.5 ± 3.1

69.8 ± 5.6*

Morphology, normal forms %

13.8 ± 8.2

12.1 ± 9.3

10.3 ± 7.6

11.5 ± 6.8

10.4 ± 9.4

12.9 ± 9.1

10.2 ± 6.9

ROS production, MFI

84.8 ± 9.3

58.5 ± 1.6

64.3 ± 3.8

79.7 ± 13.7

71.8 ± 18.6

63.3 ± 15.4

42.6 ± 6.1

Mitochondrial Membrane Potential, %

49.4 ± 8.1

49.8 ± 12.1

43.3 ± 9.2

49.5 ± 9.7

48.0 ± 6.0

47.7 ± 7.2

51.5 ± 8.6

DNA fragmentation index, %

25.6 ± 3.0

22.1 ± 4.3

24.0 ± 3.4

27.1 ± 3.5

24.7 ± 2.8

25.8 ± 4.6

23.9 ± 4.7

Membrane integrity, %

59. 9 ± 7.4

51.2 ± 7.5

53.0 ± 5.9

63.0 ± 5.8

56.7 ± 6.9

58.7 ± 4.4

57.8 ± 6.8