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Table 1 Semen parameters for the first evaluation and during the daily ejaculation period of 2 weeks. (Mean ± SEM, n = 6)

From: Influence of ejaculation frequency on seminal parameters

  First Day 2 Day 4 Day 6 Day 9 Day 11 Day 13
Abstinence, days 3-4 1 1 1 1 1 1
Volume, mL 2.5 ± 0.2 1.8 ± 0.4* 1.8 ± 0.3* 1.6 ± 0.2* 1.9 ± 0.3* 1.7 ± 0.4 * 1.9 ± 0.2*
Sperm concentration, 106/mL 121.3 ± 35.5 80.3 ± 18.7 72.1 ± 18.1 66.1 ± 15.2 75.1 ± 22.6 80.4 ± 21.5 80.3 ± 19.2
Total sperm count, ×106 276.8 ± 70.5 134.4 ± 46.6* 115.7 ± 27.8* 88.7 ± 16.2* 111.5 ± 23.6* 109.9 ± 27.3* 138.2 ± 31.4*
Total motility, % 53.83 ± 4.7 55.50 ± 10.2 54.0 ± 7.8 52.9 ± 7.0 58.5 ± 5.7 58.7 ± 4.4 51.70 ± 5.5
Progressive motility, % 47.0 ± 5.5 51 ± 11.3 43.0 ± 8.6 45.2 ± 8.0 46.6 ± 6.4 50.5 ± 5.0 40.8 ± 3.3
Vitality, % 60.2 ± 3.1 61.7 ± 8.8 53.4 ± 7.4 65.5 ± 5.3 60.2 ± 4.7 59.5 ± 3.1 69.8 ± 5.6*
Morphology, normal forms % 13.8 ± 8.2 12.1 ± 9.3 10.3 ± 7.6 11.5 ± 6.8 10.4 ± 9.4 12.9 ± 9.1 10.2 ± 6.9
ROS production, MFI 84.8 ± 9.3 58.5 ± 1.6 64.3 ± 3.8 79.7 ± 13.7 71.8 ± 18.6 63.3 ± 15.4 42.6 ± 6.1
Mitochondrial Membrane Potential, % 49.4 ± 8.1 49.8 ± 12.1 43.3 ± 9.2 49.5 ± 9.7 48.0 ± 6.0 47.7 ± 7.2 51.5 ± 8.6
DNA fragmentation index, % 25.6 ± 3.0 22.1 ± 4.3 24.0 ± 3.4 27.1 ± 3.5 24.7 ± 2.8 25.8 ± 4.6 23.9 ± 4.7
Membrane integrity, % 59. 9 ± 7.4 51.2 ± 7.5 53.0 ± 5.9 63.0 ± 5.8 56.7 ± 6.9 58.7 ± 4.4 57.8 ± 6.8
  1. MFI Mean Fluorescence Intensity, MMP Mitochondrial Membrane Potential, ROS reactive oxygen species
  2. *p < 0.05 vs. first analysis