The effect of lysophosphatidic acid during in vitro maturation of bovine cumulus–oocyte complexes: cumulus expansion, glucose metabolism and expression of genes involved in the ovulatory cascade, oocyte and blastocyst competence

Boruszewska, Dorota; Sinderewicz, Emilia; Kowalczyk-Zieba, Ilona; Grycmacher, Katarzyna; Woclawek-Potocka, Izabela

doi:10.1186/s12958-015-0044-x

Table 1 Primers used for real-time PCR

From: The effect of lysophosphatidic acid during in vitro maturation of bovine cumulus–oocyte complexes: cumulus expansion, glucose metabolism and expression of genes involved in the ovulatory cascade, oocyte and blastocyst competence

Gene	Primer sequence (5′–3′)	Fragment size, bp	GenBank accession no.
OCT4	GAGAAAGACGTGGTCCGAGTG	101	NM_174580.2
OCT4	GACCCAGCAGCCTCAAAATC	101	NM_174580.2
SOX2	TGGATCGGCCAGAAGAGGAG	89	NM_001105463.2
SOX2	CAGGCGAAGAATAATTTGGGGG	89	NM_001105463.2
IGF1R	GAGTGGAGAAATCTGCGGG	110	NM_001244612.1
IGF1R	AAATGAGCAGGATGTGGAGGT	110	NM_001244612.1
IGF2R	ACCTCCGATCCTCAATCCCA	82	NM_174352.2
IGF2R	TGTAGTTGAAGTGCCGGTCC	82	NM_174352.2
PLAC8	TTTACCGCTCTGTGCCCTTT	95	NM_001025325.2
PLAC8	CCATGTGAACTTGACCAAGCAT	95	NM_001025325.2
BCL2	GAGTTCGGAGGGGTCATGTG	203	NM_001166486.1
BCL2	GCCTTCAGAGACAGCCAGGA	203	NM_001166486.1
BAX	GTGCCCGAGTTGATCAGGAC	126	NM_173894.1
BAX	CCATGTGGGTGTCCCAAAGT	126	NM_173894.1
AREG	CTTTCGTCTCTGCCATGACCTT	100	NM_001099092.1
AREG	CGTTCTTCAGCGACACCTTCA	100	NM_001099092.1
EREG	TCACCGCGAGAAGGATGGAG	73	XM_002688367.3
EREG	GTACTGAAGACCAGGACGAGC	73	XM_002688367.3
BTC	GCCCCAAGCAGTACAAGCAT	100	NM_173896.2\|
BTC	GCCCCAGCATAGCCTTCATC	100	NM_173896.2\|
EGFR	AAAGTTTGCCAAGGGACAAG	253	XM_002696890.2
EGFR	AAAGCACATTTCCTCGGATG	253	XM_002696890.2
ADAM10	GCTGGGAGGTCAGTATGGAA	105	NM_174496.2
ADAM10	CTTTTGGCACGCTGGTGTTT	105	NM_174496.2
ADAM17	TGTTCCACCCCAGTAACGTC	73	XM_002691486.3
ADAM17	GGAAAGGGCTTGATGATGCG	73	XM_002691486.3
HAS2	CGGGGGAGATGTCCAGATTTT	120	NM_174079.2
HAS2	TGGACACATCCGAAATAAGACTG	120	NM_174079.2
PTX3	GCCGGCAGGTTGTGAAACA	93	NM_001076259.2
PTX3	GTCTCGAGTTTCATTGGTGTCA	93	NM_001076259.2
TNFAIP6	TGCTACAACCCACATGCAAA	83	NM_001007813.2
TNFAIP6	TCATTTGGGAAGCCTGGAGA	83	NM_001007813.2
PTGS2	TGGGTGTGAAAGGGAGGAAA	127	NM_174445.2
PTGS2	AAGTGCTGGGCAAAGAATGC	127	NM_174445.2
GFPT1	AAACACAGTCGGCAGTTCCA	80	NM_001109961.1
GFPT1	TGGCTACACCAATCTCAGGC	80	NM_001109961.1
GFPT2	GAGATGTGCGGAATCTTTGCC	120	NM_001076883.1
GFPT2	ACCTGCTGAGTCATAGCCTCT	120	NM_001076883.1
GLUT1	GATCCACAGAGCGCAGCC	90	NM_174602.2
GLUT1	TGTCAGCTTCTTGCTGGTGG	90	NM_174602.2
GLUT4	ATTGTGGCCATCTTTGGCTTCGTG	160	NM_174604.1
GLUT4	AACCCATGCCGATGATGAAGTTGC	160	NM_174604.1
LDHA	TCTGGATTCAGCTCGCTTCCGTTA	147	NM_174099.2
LDHA	TTCTTCAGGGAGACACCAGCAACA	147	NM_174099.2
PFKP	TCAGAGAACCGTGCCTGGAAGAAA	112	NM_001193220.1
PFKP	TGACCACAAGCTCCTTGATCTGCT	112	NM_001193220.1
GAPDH	CACCCTCAAGATTGTCAGCA	103	NM_001034034.2
GAPDH	GGTCATAAGTCCCTCCACGA	103	NM_001034034.2

Back to article page

ISSN: 1477-7827

Contact us

General enquiries: journalsubmissions@springernature.com

Reproductive Biology and Endocrinology

Contact us