The periovulatory endocrine milieu affects the uterine redox environment in beef cows

Table 2 Characteristics of the primers used for qPCR

Target gene	GenBank ID	Primer sequence (5’-3’)		Amplicon (bp)	Reference
SOD1	NM_174615.2	F	GTTGGAGACCTGGGCAATGT	151	Primer Express*
		R	TCCACCCTCGCCCAAGTCAT
SOD2	NM_201527.2	F	CCCATGAAGCCTTTCTAATCCTG	307	[47]
		R	TTCAGAGGCGCTACTATTTCCTTC		[47]
SOD3	NM_001082610.1	F	GAGAGCGAGTGTAAAGCCGT	190	PrimerQuest**
		R	CCTGGAAGAGGCACACAGAG
CAT	NM_001035386.2	F	CGCGCAGAAACCTGATGTC	150	Primer Express*
		R	GGAATTCTCTCCCGGTCAAAG
GPX4	NM_174770.3	F	TCACCAAGTTCCTCATTGACAAGA	150	Primer Express*
		R	TTCTCGGAACACAGGCAACA
PPIA	NM_178320.2	F	GCCATGGAGCGCTTTGG	70	[48]
		R	CCACAGTCAGCAATGGTGATCT
ACTB	NM_173979.3	F	GGATGAGGCTCAGAGCAAGAGA	78	[48]
		R	TCGTCCCAGTTGGTGACGAT
GAPDH	NM_001034034.2	F	GCCATCAATGACCCCTTCAT	71	[48]
		R	TGCCGTGGGTGGAATCA

Abbreviations: SOD1 Superoxide dismutase 1, SOD2 Superoxide dismutase 2, SOD3 Superoxide dismutase 3, CAT Catalase, GPX4 Glutathione peroxidase, PPIA peptidylprolyl isomerase A (cyclophilin A), ACTB actin, beta, GAPDH glyceraldehyde-3-phosphate dehydrogenase. ID: GenBank Identification, *Primer sequences obtained using Primer Express software, version 3.0 (Life technologies, Carlsbad, CA, USA), **Primer sequences obtained using PrimerQuestQM software (IDT Technologies, Coralville, IA, USA).

ISSN: 1477-7827