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Figure 6 | Reproductive Biology and Endocrinology

Figure 6

From: Activity of the porcine gonadotropin-releasing hormone receptor gene promoter is partially conferred by a distal gonadotrope specific element (GSE) within an upstream enhancing region, two proximal GSEs and a retinoid X receptor binding site

Figure 6

A third GSE within an upstream enhancing region is important for maximal basal activity of the GnRHR promoter. A, EMSAs were performed with nuclear extracts and oligonucleotides spanning -1779/-1749 bp of promoter. Along with homologous and heterologous, unlabeled oligonucleotides containing consensus binding sequences for NF-Y or SF1 were added (arrow indicates specific complex). An antibody directed against SF1 (including its DNA binding domain) or an equal mass of rabbit IgG were included (arrow indicates abrogation of complex). B, Transfections of cells with -5118pGL3, μSF1IIIpGL3 or pGL3. Unique letters indicate values that are significantly different from one another (P < 0.05).

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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