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Figure 5 | Reproductive Biology and Endocrinology

Figure 5

From: Activity of the porcine gonadotropin-releasing hormone receptor gene promoter is partially conferred by a distal gonadotrope specific element (GSE) within an upstream enhancing region, two proximal GSEs and a retinoid X receptor binding site

Figure 5

Activity of the porcine GnRHR promoter is dependent on an important upstream enhancing region. A, Cells were transfected with vectors containing sequential removal of 5′ flanking sequence in -1915pGL3 or pGL3. Unique letters indicate values that are significantly different from one another (P < 0.05). B, EMSAs performed with nuclear extracts and probes spanning the -1810/-1667 region of the porcine GnRHR promoter. Specificity of DNA-protein interactions was assessed by competition with excess homologous or heterologous DNA (arrow indicates specific complexes).

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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