Chemoablated mouse seminiferous tubular cells enriched for very small embryonic-like stem cells undergo spontaneous spermatogenesis in vitro

Table 1 Primer sequences for various transcripts used in the study

Primer	Sequence	Annealing temperature
Sca-1
F	AGAGGAAGTTTTATCTGTGCAGCCC	66°C
R	TCCACAATAACTGCTGCCTCCTGA
Gfra
F	GGCCTACTCGGGACTGATTGG	58°C
R	GGGAGGAGCAGCCATTGATTT
Prohibitin
F	GTGGCGTACAGGACATTGTG	58°C
R	AGCTCTCGCTGGGTAATCAA
Protamine
F	GGCCACCACCACCACAGACACAGGCG	66°C
R	TTAGTGATGGTGCCTCCTACATTTCC
Dazl
F	GTGTGTCGAAGGGCTATGGAT	61°C
R	ACAGGCAGCTGATATCCAGTG
PCNA
F	5′ GATGCCGTCGGGTGAATTTG 3′	55°C
R	5′ TCTCTATGGTTACCGCCTCCT 3′
SCP3
F	5′ TGTTGCAGCAGTGGGAACTGGAT 3′	68°C
R	5′ CCATCTCTTGCTGCTGAGTTTCCA 3′
18s
F	5′ GTCCCGTAGACAAAATGGTGA 3′	58°C
R	5′ TGCATTGCTGACAATCTTGAG 3′
Gapdh
F	GTCCCGTAGACAAAATGGTGA	58°C
R	TGCATTGCTGACAATCTTGAG

ISSN: 1477-7827