Beneficial effects of relaxin on motility characteristics of stored boar spermatozoa

Table 3 Effect of porcine relaxin (pRLX) on sperm viability following storage

Relaxin concentrations (ng/ml)	Day 0	Day 1	Day 2	Day 4
	Spermatozoa with intact plasma membrane (%)
0	85.5 +/− 5.5^{a, ¥}	85.5 +/− 5.5^{a, ¥}	79.0 +/− 5.5^{a, ¥}	79.5 +/− 5.5^{a, ¥}
100	87.0 +/− 5.5^{a, ¥}	90.5 +/− 5.5^{a, ¥}	87.5 +/− 5.5^{a, ¥}	80.5 +/− 5.5^{a, ¥}
	Mean fluorescence of PI in all sperm cells (RFI)
0	41.0 +/− 7.1^¥§	26.0 +/− 7.1^¥	56.5 +/− 7.1^§€	86.0 +/− 7.1^{a, €}
100	39.5 +/− 7.1^¥§	26.0 +/− 7.1^¥	52.5 +/− 7.1^§	38.5 +/− 7.1^{b, ¥§}
	Spermatozoa with intact mitochondrial membrane (%)
0	88.5 +/− 2.5^{a, ¥}	88.0 +/− 2.5^{a, ¥}	89.0 +/− 2.5^{a, ¥}	88.0 +/− 2.5^{a, ¥}
100	89.0 +/− 2.5^{a, ¥}	88.5 +/− 2.5^{a, ¥}	84.0 +/− 2.5^{a, ¥}	90.0 +/− 2.5^{a, ¥}
	Mean fluorescence of JC-1 in all sperm cells (RFI)
0	451 +/− 318^{a, ¥}	1254 +/− 318^{a, ¥}	682 +/− 318^{a, ¥}	802 +/− 318^{a, ¥}
100	583 +/− 318^{a, ¥}	1338 +/− 318^{a, ¥}	1290 +/− 318^{a, ¥}	748 +/− 318^{a, ¥}

Letters (a,b) and symbols (¥,§,€) indicate significant differences within the same column (relaxin effect) and the same row (storage effect), respectively (p < 0.05). Data are means (+/− SEM) 4 independent replicates, with 5,000 cells analyzed in each experimental condition per replicate. The global effects of relaxin on sperm membrane integrity, mean fluorescence of PI (RFI), mitochondrial membrane integrity, and mean fluorescence of JC-1 were 0.333, 0.03, 0.788, and 0.417, respectively. The global effect of storage on sperm membrane integrity, mean fluorescence of PI (RFI), mitochondrial membrane integrity, and mean fluorescence of JC-1 were 0.519, 0.004, 0.760, and 0.172, respectively.

ISSN: 1477-7827