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Table 1 Effect of porcine relaxin (pRLX) on sperm motility and velocity parameters (VAP, VSL, and VCL) following storage

From: Beneficial effects of relaxin on motility characteristics of stored boar spermatozoa

Relaxin concentrations (ng/ml)

Day 0

Day 1

Day 2

 

Proportions of total motile spermatozoa (%)

0

62.7 +/− 2.7a, ¥

58.5 +/− 2.5a, §

54.1 +/− 3.2a, €

50

65.3 +/− 2.9a, ¥

62.3 +/− 2.5ab, ¥

57.1 +/− 2.9a, §

100

65.4 +/− 2.3a, ¥

66.1 +/− 1.9b, ¥

56.8 +/− 3.1a, §

 

Proportions of progressive spermatozoa (%)

0

23.1 +/− 2.4a, ¥

30.8 +/− 2.3a, §

26.2 +/− 2.4a, ¥§

50

28.2 +/− 4.2ab, ¥

38.8 +/− 2.0b, §

32.2 +/− 2.1b, ¥§

100

32.3 +/− 1.9b, ¥

36.6 +/− 1.9b, ¥

33.4 +/− 2.5b, ¥

 

Proportions of rapid spermatozoa (%)

0

32.5 +/− 3.9a, ¥

46.2 +/− 2.6a, §

41.6 +/− 4.3a, §

50

35.1 +/− 4.8ab, ¥

48.4 +/− 3.0a, §

48.0 +/− 2.6b, §

100

40.4 +/− 2.4b, ¥

49.1 +/− 2.9a, §

48.9 +/− 2.7b, §

 

Average Path Velocity (VAP; μm/s)

0

61.4 +/− 3.1a, ¥

71.1 +/− 2.5a, §

72.7 +/− 3.3a, §

50

65.8 +/− 3.7ab, ¥

73.1 +/− 2.9a, ¥§

76.2 +/− 3.2a, §

100

69.8 +/− 2.9b, ¥

74.0 +/− 1.7a, ¥§

76.2 +/− 2.8a, §

 

Curvilinear Velocity (VCL; μm/s)

0

119.4 +/− 5.7a, ¥

142.3 +/− 4.4a, §

146.5 +/− 4.6a, §

50

120.3 +/− 4.7a, ¥

139.5 +/− 4.9a, §

145.6 +/− 4.7a, §

100

125.2 +/− 5.6a, ¥

137.5 +/− 2.9a, §

138.4 +/− 4.1a, §

 

Straight Line Velocity (VSL; μm/s)

0

48.0 +/− 1.9a, ¥

52.7 +/− 1.7a, §

53.7 +/− 2.5a, §

50

53.2 +/− 3.0ab, ¥

57.5 +/− 2.0ab, §

57.0 +/− 2.4a, §

100

57.1 +/− 2.1b, ¥

58.6 +/− 1.2b, ¥

56.9 +/− 2.3a, ¥

  1. Letters (a,b,c) and symbols (¥,§,€) indicate significant differences within the same column (relaxin effect) and the same row (storage effect), respectively (p ≤ 0.05). Data are means (+/− SEM) of 4 independent replicates, representing approximately 8,869 to 10,600 spermatozoa analyzed on a total of 48 CASA fields.