Effect of pRLX on sperm cAMP content after storage. Data are means (+/− SD) of three independent replicates, corresponding to nine individual analyses. The cAMP levels of spermatozoa in the control groups (0 ng pRLX/ml) significantly increased during storage (a,b; p < 0.05) and no significant effect of pRLX was found within the same storage day (*; p > 0.05). The insert indicates groups of spermatozoa ran independently to evaluate the effect of forskolin (250 μg/ml), used as positive control for cAMP assessment on Day 0 and Day 2. Data are mean+/− SEM and columns with different letters (a, b) indicating significant differences within the same Day.