Representative micrographs of immunofluorescence detection of RXFP1 and RXFP2 relaxin receptors in boar spermatozoa. The immunofluorescence detection of RXFP1 and RXFP2 (green FITC-staining) receptors of boar spermatozoa are shown in micrographs C and E, respectively. The micrograph A corresponds to the negative control, without a distinctive green FITC staining on spermatozoa. The bottom panel shows overlay images of visible light, blue staining of sperm nuclei (DAPI), and green FITC-staining of the Control (B), RXFP1 (D) and RXFP 2 (F) groups. Scale bars correspond to 10 μm.