Offspring subcutaneous adipose markers are sensitive to the timing of maternal gestational weight gain

Giblin, Linda; Darimont, Christian; Leone, Patricia; McNamara, Louise B; Blancher, Florence; Berry, Donagh; Castañeda-Gutiérrez, Eurídice; Lawlor, Peadar G

doi:10.1186/s12958-015-0009-0

Reproductive Biology and Endocrinology

Table 1 Ingredient composition and nutrient content of experimental diets on a meal equivalent basis (g/kg)

From: Offspring subcutaneous adipose markers are sensitive to the timing of maternal gestational weight gain

Diet type	Gestation	Lactation	Starter ^a	Link ^a	Weaner	Finisher
Wheat	0	424	ndc	ndc	455	404
Barley	893	350	ndc	ndc	225	364
Soya 50	75	160	ndc	ndc	180	200
Full fat soya	0		ndc	ndc	100	0
Soya oil	10	40	ndc	ndc	10	10
Mineral and vitamins^b	1.5	1.5	ndc	ndc	3	1
Lysine HCl^c	0.5	2	ndc	ndc	4	3
DL-Methionine^c	0	0.7	ndc	ndc	2	0.8
L-Threonine^c	0	0.8	ndc	ndc	1.5	1
Di-calcium phosphate	5	5	ndc	ndc	5
Limestone flour	11	12	ndc	ndc	11	13
Salt	4	4	ndc	ndc	3	3
Pulmotil^d	0	0	ndc	+	0	0
Phytase 5000,^e IU/g	0.1	0.1	ndc	ndc	0.1	0.1
Chemical composition, g/kg
Dry matter	871	873	870	870	872	870
Crude Protein	132	158	200	200	196	178
Fat	31	56	90	75	43	27
Crude fibre	45	35	25	30	36	37
Ash	44	46	60	60	50	44
Lysine^f	6.2	9.1	16	15	13.1	11.1
Digestible energy,^f MJ/kg	13	14.2	16.3	15.4	14.1	13.7

Sow, weaner and finisher diets were manufactured onsite.
Starter and link diets were manufactured by Devenish Nutrition (Belfast, Northern Ireland).
^aCommercial diets for which the ingredient composition was not disclosed (ndc).
^bProvided per kilogram of complete diet.
Gestation and lactation diets: Cu, 38 mg; Fe, 70 mg; Mn, 62 mg; Zn, 80 mg; I, 0.6 mg; Se, 0.2 mg; vitamin A, 10000 IU; vitamin D₃, 1000 IU; vitamin E, 100 IU; vitamin K, 2 mg; vitamin B₁₂, 15 μg; riboflavin, 5 mg; nicotinic acid, 12 mg; pantothenic acid, 10 mg; choline chloride, 500 mg; Biotin, 200 μg; Folic acid, 5 mg; vitamin B₁, 2 mg and vitamin B₆, 3 mg.
Weaner diet: Cu, 175 mg; Fe, 140 mg; Mn, 47 mg; Zn, 120 mg; I, 0.6 mg; Se, 0.3 mg; vitamin A, 6000 IU; vitamin D₃, 1000 IU; vitamin E, 100 IU; vitamin K, 4 mg; vitamin B₁₂, 15 μg; riboflavin, 2 mg; nicotinic acid, 12 mg; pantothenic acid, 10 mg; choline chloride, 250 mg; vitamin B₁, 2 mg; vitamin B₆, 3 mg; and endox, 60 mg.
Finisher diet: Cu, 100 mg; Fe, 40 mg; Mn, 31 mg; Zn, 80 mg; I, 0.3 mg; Se, 0.2 mg; vitamin A, 2000 IU; vitamin D₃, 500 IU; vitamin E, 40 IU; vitamin K, 4 mg; vitamin B₁₂, 15 μg; riboflavin, 2 mg; nicotinic acid, 12 mg; pantothenic acid, 10 mg; vitamin B₁, 2 mg and vitamin B₆, 3 mg.
^cSynthetic amino acids.
^dLink diet contained 200 mg Tilmicosin per kg of feed provided from Pulmotil G100 (Eli Lilly, Basingstoke, Hampshire, England).
^eSow, weaner and finisher diets contained 500 FTU phytase per kg finished feed from Natuphos 5000 (BASF, Ludwigshafen, Germany).
^fCalculated values.

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