Skip to main content
Figure 3 | Reproductive Biology and Endocrinology

Figure 3

From: Characterization and possible function of glyceraldehyde-3-phosphate dehydrogenase-spermatogenic protein GAPDHS in mammalian sperm

Figure 3

Biochemical confirmation of the Hs-8 target specificity. Immunodetection of human sperm extracts and purified recombinant GAPDHS-GST tagged protein with MoAb Hs-8 (A) and with commercial mouse monoclonal antibody against GAPDHS (B). Arrows indicate the GAPDH protein labelled with anti-GAPDHS antibody. St – protein standard, hu sperm – human sperm cell lysate, GAPDHs – recombinant purified GAPDHS protein, hu sperm (SDS) – SDS extract of human sperm cells, hu sperm (RHB) – human sperm cell proteins extracted with RHB buffer.

Back to article page

Reproductive Biology and Endocrinology

ISSN: 1477-7827

Contact us