Figure 2

Formation of fetal germ cells, granulosa cells, and follicular development in midpregnancy human fetal ovary. A) CD14 MDC interacts (arrowheads) with an OSC (yellow asterisk and dotted circle) prior to the asymmetric division. B) Numerous MHC-I depleted fetal germ cells (blue asterisks and dashed circles) originating by asymmetric divisions (arrowhead) from MHC-I+ OSCs (yellow asterisks and dotted circles). C) Asymmetric division (blue arrowhead) is followed by a symmetric division of MHC I depleted germ cells (red asterisks and arrowhead). This is followed by the development of ameboid shape fetal moving germ cell (mgc - dashed line, no hematoxylin counterstain) entering adjacent ovarian cortex. Asymmetric division is accompanied by CD8 (D) and DR+ (E) T cell. F) CD14 MDC (arrowhead) accompanies symmetric division of germ cell during meiosis I telophase. G) Development of primitive granulosa cells (pgrc, note lower CK expression) from ovarian stem cells between mesenchymal cell cords (mcc). H) DR+ MDC accompany (arrows) fetal growing follicle (gf) but not the resting follicle (rf). Inset shows association of Thy-1+ pericytes (arrowhead) with a growing but not resting follicles. Bar in A for A-F. Adapted from Ref. [25] with a permission: © Springer US.