Figure 3

Invasion assay. A. Representative images of invaded inserts showing the effect of BK, the B1R agonist LDBK, and of preincubation with the antagonists of the B2 and B1 receptors, HOE-140/BDZ and AB1R respectively, on the invasive capacity at 18 hours, evaluated by the number of cells focussed on the underside of the filter. Blurry cells correspond to non-invading cells in the upper surface of the filter. B. Effect of BK and LDBK on the invasive capacity of HTR-8/SVneo cells analyzed at 18 hours, with and without preincubation with the B2R antagonists (HOE-140/BDZ] and the AB1R. N = 4 for each condition. Mean ± SE; *p < 0.05 vs. control and HOE-140. Bar = 100 μm.