The effect of fractalkine on the hCG stimulation of transcript levels for different steroidogenic enzymes in cultured granulosa cells. Isolated granulosa cells were cultured with 20 ng/ml hCG and/or 100 ng/ml fractalkine for 48 h. Transcript levels of StAR (A), CYP11A (B) and 3β-HSD (C) were quantitated using real-time RT-PCR. Data are represented as the mean ± SE of three independent experiments. A Transcript levels of StAR; B Transcript levels of CYP11A; C Transcript levels of 3β-HSD. *, P < 0.05 compared to cells treated with hCG alone.