Figure 1

Flow cytometry analysis of decidual leukocytes. Comparison of typical subset-markers of NK cells (CD3^-, CD56⁺, CD16^+/-) and the non-typical dNK cells marker (CD9⁺). A-D) Representative FACS for two-color staining for decidual leukocytes. E-G) Representative FACS for three-color staining for decidual leukocytes. H-K) Representative FACS two-color staining for peripheral blood mononuclear cells (PBMC). L-M) Representative FACS three-color staining for PBMC. A, E, H, L) negative cells; B, I) upper left quadrants (UL): CD56⁺ CD3^- cells; C, J) UL: CD56⁺ CD16^- cells; upper right quadrants (UR): CD56⁺ CD16⁺ cells; D, K) UR: CD56⁺CD9⁺ cells. F, M) R1 and R2, gate for CD3^- cells; G, N) CD56⁺CD9^+/- expression on CD3^- cells.