Figure 5

Testosterone concentration in the ovarian follicular fluid and testosterone secreted in vitro in follicular wall. In A is shown the testosterone concentration in the follicular fluid and in B is shown testosterone secreted following stimulation with a β-receptor agonist (isoproterenol) and with hCG (B). Follicular wall pieces were incubated for 3 h in 2 ml of Krebs-Ringer bicarbonate buffer (basal), 10 μ M isoproterenol (ISO) or 5 IU hCG. Results are expressed as ng testosterone per ml of incubation medium per mg of tissue during 3 h. Each bar represents the mean ± SEM of four independent observations per group. *= p < 0.01 vs Small antral (SA); **= p < 0.01 vs Preoulatory follicles (PO). a, b = p < 0.05 vs. basal (SA, PO and Cystic).