PreImplantation Factor (PIF) correlates with early mammalian embryo development-bovine and murine models

Table 2 Anti-PIF-antibody reduces mouse embryos blastocyst development

Anti-PIF Mab Titration N =		24		26		36		27		30		19
	47000 ng/mL	%	4700 ng/mL	%	470 ng/mL	%	47 ng/mL	%	Neg. Contr.	%	Isotype Contr.	%
Blastocyst	5	0.21	8	0.31	11	0.32	11	0.41	23	0.77	13	0.68
Eblast	2	0.08	2	0.08	7	0.21	4	0.15	5	0.17	3	0.16
Morula	2	0.08	0	0.00	4	0.12	0	0.00	2	0.07	2	0.11
6-8 cell	0	0.00	7	0.27	2	0.06	3	0.11	0	0.00	0	0.00
2-4 cell	11	0.46	5	0.19	11	0.32	8	0.30	0	0.00	1	0.05
AT > Morula	0	0.00	2	0.08	0	0.00	0	0.00	0	0.00	0	0.00
AT < Morula	4	0.17	2	0.08	1	0.03	1	0.04	0	0.00	0	0.00

Mouse 2cell embryos were collected from mice and cultured with increasing concentrations of anti-PIF-antibody added to the culture media. (N = 19-36/group). Results were compared with isotype antibody and medium alone used as controls. Data is expressed as number of embryos in each developmental stage. Inhibitory effect of anti-PIF-antibody was dose dependent. Data analyzed by using (2-way contingency table, multiple groups, (X²; P = 0.01).

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