Figure 2

PIF detection in culture medium was associated with mouse embryo viability. Mouse embryos were cultured in groups up to the morula stage medium collected and further cultured up the blastocyst stage collecting the medium. PIF levels were compared with cultured atretic embryos and medium control. Data are expressed mean+/-SEM. PIF levels were significantly higher in both morulae and blastocysts (p = 0.05 and p = 0.01) respectively, as compared to similar levels obtained in atretic and medium alone used as control.