Figure 3

PMP22 expression increased binding on laminin. (A) 5 × 10⁴ cells (HEC-1A/PMP22, HEC-1A/Vector, HEC-1A/scrambled siRNA control, and HEC-1A/PMP22 siRNA) were incubated in serum-free media at 37°C for 30 min in wells coated with different extracellular matrices. Absorbance was measured at 595 nm. Values are represented as the mean ± SEM. for triplicate wells (*p < 0.05; Student's unpaired t test). A representative experiment is shown; three independent experiments yielded similar results. (B) In order to confirm that laminin binding was α6 integrin dependent, HEC-1A/PMP22 cells were incubated with indicated concentrations of α6 or α2 (control) integrin antibodies. Samples were then plated on laminin for 30 min, and analyzed as above.