Figure 2

PMP22 expression differentially regulates select integrin pairs. The surface expression of α6, αvβ3, or α2 integrin in HEC-1A/PMP22, HEC-1A/Vector, or HEC-1A/PMP22 siRNA cells was determined using flow cytometry. The MFI was quantitated and tabulated in the top corner of each histogram. Upregulation of PMP22 promoted the surface expression of α6 integrin, while slightly reducing αvβ3 integrin. Reciprocal effects were seen with the PMP22 specific siRNA. In contrast, no change in α2 integrin expression was observed when PMP22 expression was modulated. The experiment was repeated three times, and a representative experiment is shown. Statistical differences between α6 integrin (B) and αvβ3 (C) expression were determined between the three cell lines. *, p < 0.05; Student's unpaired t test.