Table 1 Characteristics of targets and primary antibodies used in the study

β-actin

Sheep IgG^a

2.0 μg/ml

Internal normalization control, used in WB

Cytokeratin 7

Mouse IgG^b

6 μg/ml

Epithelial cell marker, used in ICC

Cytokeratin 18 neo-epitope (CK18f)

Mouse IgG^c

1:50 (WB); 1:20 (ICC)

Early apoptosis marker, used in WB and ICC

βhCG

Rabbit IgG^c

4 μg/ml (WB); 10 μg/ml (ICC)

CTB differentiation marker, used in WB and ICC

hPL Goat IgG^d

3 μg/ml (WB); 7 μg/ml (ICC)

CTB differentiation marker, used in WB and ICC

Vimentin

Mouse IgG^a

3 μg/ml

Cytoskeletal protein marker for fibroblasts, used in ICC

Vitronectin receptor, Integrin αVβ3 (CD51)

Mouse IgG^e

10 μg/ml

Invasive CTB marker, used in ICC

von Willebrand factor

Rabbit IgG^c

25 μg/ml

Endothelial cell marker (in Weibel-Palade bodies), used in ICC