Figure 4

Representative Western blot analysis of immunopositive levels of βhCG, hPL and cytokeratin 18 neo-epitope (CK18f) in cytotrophoblast cells grown in primary culture. Cell lysates of isolated villous trophoblast cells grown on collagen biomatrix for 24 h, 48 h and 96 h with or without AMA (1000 ng/ml) were electrophoretically separated and subjected to immunoblot analysis using β-actin as the internal control.