Table 1 Primer sequences and conditions used for quantitative PCR analysis
Gene Name | Primer Sequence (5'-3') | Size (bp) | Anneal temp (°C) | Extension time (s) | Mg2+ (mM) |
|---|---|---|---|---|---|
PC5/6 | F: TCTGACCTGGAGAGACGTAC R: TGTCTTGATATGTCGATCTG | 221 | 55 | 9 | 3.0 |
18S | F: CGGCTACCACATCCAAGGAA R: GCTGGAATTACCGCGGCT | 187 | 64 | 8 | 3.0 |