Figure 1

RT-PCR confirmation of PC5/6 mRNA expression in the rabbit uterus. (A) Agarose gel electrophoresis of RT-PCR products of PC5/6 (220 bp) and 18S (187 bp) respectively. Two negative controls are: -RT, no reverse transcription enzyme in RT reaction; -RNA, no RNA in RT reaction. +RT+RNA, RT reaction contained RNA and reverse transcription enzyme. M, DNA marker. (B) Comparison between sequences of rabbit PC5/6 mRNA (Rabbit-PC5/6) and RT-PCR amplified uterine PC5/6 product (PCR-PC5/6).