Table 1 Proteomic studies on mature human spermatozoa
Authors | Study | Proteomic Technique | Outcome |
|---|---|---|---|
Johnston et al. 2005 [37] | First extensive analysis of human sperm proteome | LC-MS/MS | 1760 proteins identified |
Goal to provide a reference 2D map of the mature human sperm proteome | MALDI-TOF | 131 proteins identified | |
Ficarro et al. 2003 [38] | Phosphoproteome analysis of capacitated human sperm | LC-MS/MS | 60 proteins identified |
Domagala et al. 2007 [32] | Identification of sperm immunogenic antigens | LC-MS | 35 proteins identified |
Secciani et al. 2009 [33] | Protein profile of capacitated versus ejaculated human sperm | MS/MS | 116 proteins identified |
Shetty et al. 2001 [39] | Identification of immunocontraceptive candidates | MALDI-MS | 8 proteins identified |
Bohring et al. 2001 [34] | Isolation and identification of sperm membrane antigens | MALDI-MS | 18 proteins identified |
Baker et al. 2007 [29] | Identification of proteins in human sperm using LC-MS/MS analysis | LC-MS/MS | 1056 proteins identified |
Lefievre et al. 2007 [35] | Identify targets for S-nitrosylation in human sperm | LC-MS/MS | 240 proteins identified |
Li et al. 2007 [28] | Establish high-resolution 2-D map of human sperm proteins | MALDI-MS | 16 proteins identified |