Biochemical isolation and purification of ovulation-inducing factor (OIF) in seminal plasma of llamas

Table 2 Ovulation-inducing effect of protein fractions of llama seminal plasma (full experiment)

	PBS	SP	Fraction A	Fraction B	Fraction C₂
Follicle diameter (mm) at treatment* (mean ± SEM)	8.1 ± 0.4	8.5 ± 0.4	8.8 ± 0.3	9.5 ± 0.6	8.9 ± 0.3
Ovulation rate (%)	0/10^a (0%)	10/11^b (91%)	0/10^a (0%)	2/11^a (18%)	10/11^b (91%)

Female llamas (n = 10-11 per group) were given whole seminal plasma (SP, positive control), Fractions A or B (isolated by hydroxylapatite column chromatography), Fraction C₂ (isolated by gel filtration chromatography), or phosphate buffered saline (PBS, negative control).
* No significant difference among groups
^a,b Proportions with different superscripts are different (P < 0.001)

ISSN: 1477-7827