Figure 2
Analysis of expression of genes encoding PR, its cofactors and P 4 target genes in CL during different stages of the luteal phase in monkeys. A) Real time RT-PCR fold change in expression (represented as mean ± SEM; n = 3 animals/stage of the luteal phase) of various genes associated with PR signaling and its target genes. The fold expression change at early stage was set as 1 and fold changes in other stages were expressed in relation to the early stage. Bars with different letters indicate significant (P < 0.05) change in the expression of the individual gene examined. B) Immunoblot analysis of PR, NCOA1, NCOA2 and NCOA3 expression in CL collected during various stages of the luteal phase. Immunoblots were analyzed by densitometry, and the densitometric value of early stage CL for each protein was set as 1 and values at other stages of CL were expressed in relation to the early stage. For NCOA1 protein, the upper band indicated by asterisk was considered for densitometric analysis. The statistical analysis details for densitometric values for each protein are provided in the results section.