Western blot analysis of pig AKR1C1 protein in the ovary during the estrous cycle and pregnancy. A. Pig ovaries on days 0, 5, 10, and 15 of the estrous cycle were obtained by laparotomy under general anesthesia. B. Uteri were collected on days 12, and 30 of pregnancy. The proteins were transferred onto a PVDF membrane. Proteins on the blot were detected with rabbit anti-bovine 20α-HSD antibody, followed by staining with secondary antibody linked to anti-rabbit IgG-peroxide. M, marker; p-part, before parturition.