Figure 3

ICI 182,780 revert E2-inhibitory effect in the release of a-defensins 1-3 by DCs. CD14⁺ cells isolated from fresh blood were cultured for 5 days in the presence of IL-4 and GM-CSF to differentiate into DCs. The cells were treated with 10, 100 and 1000 nM of ICI 182,780 for the last 3 days of the differentiation process. (A) Levels of ERa measured by RT-PCR in DCs treated or not with ICI 182,780 in the presence of E2 (10 ug/ml) for 24 h. The data presented as mean ± SD are representative of three independent experiments. (B) Percentage of reduction induces by E2-treatment in the levels of a-defensins 1-3 secreted by MDDCs and mDCs pre-treated or not for 3 days (MDDCs) or 24 h (mDCs) with 1000 nM of ICI 182,780 or DMSO, prior to 24 h of Estrogen or Mock treatment.. The data presented as mean ± SD are representative of three independent experiments. (C) Percentage of CD11c+CD14+ cells measured by FACS after 5 days of differentiation in the presence of ICI 182,780 or DMSO during the last 3 days of the process. These data are representative of three independent experiments.