Western blot analysis of p97/VCP and Jab1/CSN5 in the developing rat testis (A) and epididymis (B). A: p97/VCP (97 kDa) and Jab1/CSN5 (38 kDa) were detected by western blotting. β-actin (43 kDa) was used as loading control. Immunoblot bands were quantified by an optical densitometer. The OD (optical density) values of the p97/VCP and Jab1/CSN5 bands were normalised to the OD values of β-actin bands. The data in the graphs are presented as the means ± SEM. The expression of p97/VCP and Jab1/CSN5 is significantly increased from days 5 to 30 in the testis (Asterisks indicate, p < 0.05); however, no differences are observed between days 30 and 60. B: p97/VCP and Jab1/CSN5 expression levels are significantly higher in the CT epididymis than the CS and CA epididymis at days 30 and 60. Asterisks indicate p < 0.05, days 5 CS vs. days 5 CA and CT epididymis, days 30 and 60 CT vs. days 30 and 60 CA and CS epididymis. CT: caput, CS: corpus, CA: cauda.