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Figure 2 | Reproductive Biology and Endocrinology

Figure 2

From: Establishment of an immortalized human endometrial stromal cell line with functional responses to ovarian stimuli

Figure 2

Induction of decidualization in KC02-44D cells. KC02-44D cells were treated with the indicated combinations of 10-8 M E2, 10-6 M MPA, and 0.125 mM dibutyryl cAMP for 6 days to assess their decidualization capacity. (a) Phase-contrast microphotographs of living KC02-44D cells at passage number 17 (scale bars = 100 μm). (b) Expression of decidualization marker IGFBP-1 in KC02-44D cells at passage number 15 (ELISA). The data represent the mean ± SEM of triplicate measurements. *P < 0.05 and ***P < 0.001 by Tukey's test. (c) Expression of the ER (RT-PCR analysis) and PR (Western blot analysis) in KC02-44D cells at passage numbers 15 and 16. β-Actin was used for normalization.

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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