Figure 4
Effect of 17β-E 2 on the proliferation and formation of large cloning efficiency of SP cells. Percentage and large cloning efficiency of SP cells treated with 17β-E2 and 17β-E2 +ICI182780 (Faslodex, inhibitor of ESR1) for 24 h. Figure 4A. SP cells were pretreated with 17β-E2 and 17β-E2 +ICI182780 at different concentration (10-8 to10-6M) for 24 h, after which the cell viability was estimated by the MTT assay. n = 4 experiments. * Compared with control, p < 0.05. Figure 4B. Distribution of colony size of SP cells cultured in phenol red-free sera medium for 15 days. The number of cells per colony from 344 colonies was counted, with the data generated used to discriminate small and large colonies, as shown by the vertical line at 70 cells. Hematoxylin staining of small clone (left) and large clone (right). Scale bar, 100 μm. Figure 4C. Large cloning efficiency of SP cells treated with 17β-E2 and 17β-E2 +ICI182780 at different concentration (10-8 to10-6M) for 15 days. #Compared to control, and *P < 0.05.