Figure 4
From: Effect of estrogens on boar sperm capacitation in vitro
Differences in the number of capacitated sperm in control and experimental samples influenced by 1 μM E2 in 8 individual animals. Sperm samples from 8 individual animals were capacitated with 1 μM E2 and ethanol (control). Sperm were collected after 120 min of capacitation and analyzed by CTC and ACR.2 immunofluorescence. Differences were analyzed by Mann-Whitney U test *P < 0.05, **P < 0.01, ***P < 0.001.