Figure 2

VEGF-D immunostaining in the mouse uterus. Representative photomicrographs (A-C) and semi-quantitative evaluation (D-F) of VEGF-D immunostaining in the mouse uterus. Representative photomicrographs from a normal cycling (estrous cycle) mouse (A), from a mouse in the early pregnancy (pre-implantation (B), and from an ovariectomised mouse (C). Inset in (A): isotyped-matched control. Ep: luminal epithelium, G: gland, M: myometrium, S: endometrial stroma. Semi-quantitative evaluation of VEGF-D (D-F) immunostaining in luminal epithelium, glandular epithelium, endometrial stroma and myometrium from the uterus of mice during the estrous cycle (D, n = 7-9), early pregnancy (E, days 1-4, n = 4-5) and in ovariectomised mice (F) following vehicle (n = 7), estradiol-17β (E × 24 h, n = 7), progesterone (P, n = 7), or progesterone following estradiol-17β priming (E + P, n = 8). Data are illustrated as means ± SEM. Groups that do not share a letter in common are significantly different (P < 0.05)