Figure 7
From: Arsenic abrogates the estrogen-signaling pathway in the rat uterus
Regulation of G1 cell cycle proteins by arsenic in rats. Female rats were treated with arsenic at a dose of 4 μg/ml for 28 days. Cyclin D1 and CDK4 expressions were analyzed both at the mRNA levels (a) and protein levels (b), using GAPDH and β-tubulin as loading controls, respectively. It was observed that arsenic downregulated both cyclin D1 and CDK4 (white bars) in the uterus of Sprague-Dawley rats, as compared to the control animals (black bars). The data shown is a representative of the experiments carried out in triplicate.