Figure 4
Dose dependent effects of naltrindole and DPDPE on capacitation, AR, vitality and mitochondrial activity: Different concentrations of DPDPE and naltrindole affect the number of capacitated (Panel a), AR (Panel B), dead (Panel C) equine sperm cells and those with active mitochondria (Panel D) after 4.5 h of incubation. Data are reported as mean ± SD, n = 6. The statistical analysis conducted by Chi square test, after comparison with the control condition at the same time point, was considered significant for p < 0.01. a = p < 0.01; b = p < 0.05 and c = p < 0.001. Nal-4 = 10-4 M naltrindole; Nal-5 = 10-5 M naltrindole; Nal-6 = 10-6 M naltrindole; Nal-7 = 10-7 M naltrindole; DPDPE-5 = 10-5 M DPDPE; DPDPE-6 = 10-6 M DPDPE; DPDPE-7 = 10-7 M DPDPE.