Figure 3
From: Effect of high intratesticular estrogen on global gene expression and testicular cell number in rats
Validation of microarray results using real time RT-PCR. Six genes were randomly selected. Data are represented as expression fold change calculated as a ratio (reference/target) as detailed in the Materials & Methods. Relative expression of each gene in the control was designated as 100, and values are mean ± SEM. '*' represents a statistically significant difference (p < 0.05) between 17β-estradiol treated rat testis and the control.