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Table 2 Human SIZP mediated induction of acrosome reaction in capacitated human sperm in presence or absence of various pharmacological inhibitors.

From: Delineation of downstream signalling components during acrosome reaction mediated by heat solubilized human zona pellucida

Treatment

Percent induction of acrosomal exocytosis (Mean ± SEM)

Statistical significance

Experiment I a

  

PBS control

14.5 ± 0.5

 

PBS + SIZP

31.1 ± 1.2

p = 0.04*b

EGTA (8 mM) + SIZP

16.2 ± 0.9

p = 0.001*c

Pertussis toxin (0.1 μg/ml) + SIZP

21.1 ± 0.8

p = 0.02*c

H-89 (20 μM) + SIZP

19.8 ± 0.9

p = 0.02*c

Experiment II a

  

Alcohol control

14.5 ± 0.7

 

Alcohol + SIZP

28.9 ± 1.0

p = 0.0004*b

Picrotoxin (50 μM) + SIZP

23.4 ± 0.5

p = 0.005*c

Picrotoxin (100 μM) + SIZP

21.3 ± 0.5

p = 0.0007*c

Experiment III a

  

DMSO Control

15.7 ± 1.4

 

DMSO + SIZP

36.0 ± 1.4

p = 0.0001*b

Chelerythrine chloride (2 μM) + SIZP

21.5 ± 1.4

p = 0.006*c

Chelerythrine chloride (3 μM) + SIZP

21.2 ± 2.4

p = 0.007*c

Wortmannin A (50 nM) + SIZP

22.3 ± 0.6

p = 0.0001*c

Wortmannin A (100 nM) + SIZP

25.0 ± 1.7

p = 0.008*c

Herbimycin A (10 μM) + SIZP

21.0 ± 1.2

p = 0.012*c

Herbimycin A (15 μM) + SIZP

19.1 ± 0.9

p = 0.02*c

  1. aCapacitated human sperm (1 × 106) pre-treated with or without respective inhibitor were incubated with SIZP (5 zonae) for 60 min
  2. bValues are statistically significant as compared to PBS control
  3. cValues are statistically significant as compared to SIZP
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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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