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Table 2 Human SIZP mediated induction of acrosome reaction in capacitated human sperm in presence or absence of various pharmacological inhibitors.

From: Delineation of downstream signalling components during acrosome reaction mediated by heat solubilized human zona pellucida

Treatment Percent induction of acrosomal exocytosis (Mean ± SEM) Statistical significance
Experiment I a   
PBS control 14.5 ± 0.5  
PBS + SIZP 31.1 ± 1.2 p = 0.04*b
EGTA (8 mM) + SIZP 16.2 ± 0.9 p = 0.001*c
Pertussis toxin (0.1 μg/ml) + SIZP 21.1 ± 0.8 p = 0.02*c
H-89 (20 μM) + SIZP 19.8 ± 0.9 p = 0.02*c
Experiment II a   
Alcohol control 14.5 ± 0.7  
Alcohol + SIZP 28.9 ± 1.0 p = 0.0004*b
Picrotoxin (50 μM) + SIZP 23.4 ± 0.5 p = 0.005*c
Picrotoxin (100 μM) + SIZP 21.3 ± 0.5 p = 0.0007*c
Experiment III a   
DMSO Control 15.7 ± 1.4  
DMSO + SIZP 36.0 ± 1.4 p = 0.0001*b
Chelerythrine chloride (2 μM) + SIZP 21.5 ± 1.4 p = 0.006*c
Chelerythrine chloride (3 μM) + SIZP 21.2 ± 2.4 p = 0.007*c
Wortmannin A (50 nM) + SIZP 22.3 ± 0.6 p = 0.0001*c
Wortmannin A (100 nM) + SIZP 25.0 ± 1.7 p = 0.008*c
Herbimycin A (10 μM) + SIZP 21.0 ± 1.2 p = 0.012*c
Herbimycin A (15 μM) + SIZP 19.1 ± 0.9 p = 0.02*c
  1. aCapacitated human sperm (1 × 106) pre-treated with or without respective inhibitor were incubated with SIZP (5 zonae) for 60 min
  2. bValues are statistically significant as compared to PBS control
  3. cValues are statistically significant as compared to SIZP